Additional Sample Sheet Fields for Autolaunch

Autolaunch-compatible sample sheets contain the following fields specific to autolaunch configuration. For more information on DRAGEN TSO 500 sample sheet requirements, refer to DRAGEN TruSight Oncology 500 Analysis v2.1.1 (Local) User Guide (Document # 200019138).

Parameter

Details

Required

Cloud_TSO500S_Data

Sample_ID

The unique ID to identify a sample. The sample ID is included in the output file names. Sample IDs are not case sensitive. Sample IDs must have the following characteristics:

• Unique for the run.
• 1–40 characters.
• No spaces.
• Alphanumeric characters with underscores and dashes. If you use an underscore or dash, enter an alphanumeric character before and after the underscore or dash. eg, Sample1-T5B1_022515.
• Cannot be called all, default, none, unknown, undetermined, stats, or reports.
• Must match a Sample_ID listed in the TSO500S_Data section.
• Illumina recommends that the sample ID be based on the pairID. eg, <PairID>-DNA,<PairID>-RNA.
• Each sample must have a unique combination of Lane (if applicable), sample ID, and index ID or the analysis will fail.

Yes

Index_ID

The index ID used for the sample. Must be unique per sample unless samples are assigned different lanes. Mismatches between the samples and index primers can cause incorrect result reporting due to loss of positive sample identification. Enter sample IDs and assign indexes in the sample sheet before beginning library preparation. Valid indexes for DNA samples are:

• UP1–UP16
• CP1–CP16
• UDP0001–UDP0192 (Used with TSO500HT)

Valid Indexes for RNA Samples are:

• UP1–UP16
• UDP0001–UDP0192 (Used with TSO500HT)

Yes

Sample_Type

Enter DNA or RNA. For HRD samples, this parameter must be DNA.

Yes

Pair_ID

A unique ID that links DNA and RNA from the same biological sample from the same individual. PairID shares, at most, one DNA and one RNA sample per run. eg, if a Sample_ID is TestSample1-DNA for DNA and TestSample1-RNA for RNA, the Pair_ID TestSample1 will link these samples that are on different rows in the sample sheet together. If the pair ID is associated with more than one DNA or RNA sample, the analysis fails.

Yes

Sample_Feature

For DNA samples that have undergone HRD enrichment, enter HRD in this column of the sample sheet. If the sample has not undergone HRD enrichment, leave the field empty.

Only required for HRD-enriched samples.

Sample_Description

Must meet the following requirements:

• 1–50 characters.
• Alpha numeric characters with underscores, dashes and spaces. If you enter a underscore, dash, or space, enter an alphanumeric character before and after. eg, Solid-FFPE_213.

No

Cloud_TSO500S_Settings

SoftwareVersion

The TSO500S software version

No

StartsFromFastq

Set the value to TRUE or FALSE.

If autolaunching from BCL files, this must be set to FALSE.

Yes

Cloud_Data

Sample_ID

The same sample ID used in the Cloud_TSO500S_Data section.

No

ProjectName

The BaseSpace project name.

No

LibraryName

Combination of sample ID and index values in the following format: sampleID_Index_Index2.

No

LibraryPrepKitName

The Library Prep Kit used.

No

IndexAdapterKitName

The Index Adapter Kit used.

No

Cloud_Settings

GeneratedVersion

The cloud GSS version used to create the sample sheet. Optional if manually updating a sample sheet.

No

CloudWorkflow

Ica_workflow_1

Yes

Cloud_TSO500_Pipeline

This value is a universal record number (URN). The valid values are:

• Solid—urn:ilmn:ica:pipeline:ba62b710-0cdc-4bfd-a157-9d90c62e524a#DRAGEN_TSO500_Solid_non-HRD_RUO_v2-1-1_3_8_2023_Official_Analysis_Software_Pipeline
• Solid HRD—urn:ilmn:ica:pipeline:8054bfa4-1d76-4c86-aaca-e340dc1a369c#DRAGEN_TSO500_Solid_HRD_RUO_v2-1-1_3_8_2023_Official_Analysis_Software_Pipeline

Yes

BCLConvert_Pipeline

The value is a URN in the following format: urn:ilmn:ica:pipeline:<pipeline-ID>#<pipeline-name>

No