Command Line Options

Option

Required

Description

--build-hash-table

Yes

Set to true. If it is set to false the pipeline will not use its end-to-end mode, and it will stop at the generation of the 3 intermediate files.

--ht-graph-vcf-list

Yes

Path to the text file containing the list of VCF files to be used to build the custom multigenome hash table

--ht-reference

Yes

Path to the reference genome FASTA file. Note: support only for human genome builds CHM13, hg38, hg19 and hs37d5.

--ht-graph-bed

Yes

Path to the graph bed file

--ht-mask-bed

Yes

Path to the mask bed file

--output-directory

Yes

Specify the directory where all related hash table files will be written

The custom reference hash table pipeline will return an error if options --ht-alt-liftover or --ht-allow-mask-and-liftover are specified.