BaseSpace Sequence Hub Overview

BaseSpace Sequence Hubis the Illumina cloud computing environment.

Log in to BaseSpace Sequence Hub when you set up the sequencing run. When using BaseSpace Sequence Hub, you have the additional option to store run data locally. For more information, refer to Configure Run Settings.

When the sequencing run begins, the icon changes to indicate that the MiSeq is connected to BaseSpace Sequence Hub. Data files start transferring to the specified location. Data files are encrypted in transit, decrypted during analysis, and then encrypted again when stored.

BaseSpace Sequence Hub automatically disconnects from the MiSeq at the end of the run or when all RTA analysis files have been successfully transferred. If the internet connection is interrupted, analysis files continue to upload after the connection is restored, starting from the point where the interruption occurred.

When the last base call file is uploaded to BaseSpace Sequence Hub, secondary analysis of the data begins. The same analysis workflows supported by on-instrument analysis using Local Run Manager are available on BaseSpace Sequence Hub.

You can connect toBaseSpace Sequence Hub at basespace.illumina.com. Log in using your MyIllumina account login. For more information on BaseSpace Sequence Hub, refer to the BaseSpace Sequence Hub support pages on the Illumina website.