Reagent Cartridge

The MiSeq reagent cartridge is a single-use consumable consisting of foil-sealed reservoirs prefilled with clustering and sequencing reagents sufficient for sequencing one flow cell.

Each reservoir on the cartridge is numbered. Sample libraries are loaded onto the cartridge in position 17, which is labeled Load Samples.

This set of reagents contains potentially hazardous chemicals. Personal injury can occur through inhalation, ingestion, skin contact, and eye contact. Ventilation should be appropriate for handling of hazardous materials in reagents. Wear protective equipment, including eye protection, gloves, and laboratory coat appropriate for risk of exposure. Handle used reagents as chemical waste and discard in accordance with applicable regional, national, and local laws and regulations. For additional environmental, health, and safety information, refer to the SDS at support.illumina.com/sds.html.

Reserved Reservoirs

Reagent Cartridge with Numbered Reservoirs

Reagent Cartridge Reservoirs

Position

Reagent Name

Description

8

LDR

Denaturation Reagent (contains formamide)

17

Reserved

Load Sample (Reserved for sample libraries)

18

Reserved

Reserved for custom Read 1 primer [Optional]

19

Reserved

Reserved for custom Index Read primer [Optional]

20

Reserved

Reserved for custom Read 2 primer [Optional]

For more information about using custom primers on the MiSeq reagent cartridge, refer to Custom Primers.