Start a Manual Run (Import Sample Sheet)

Format Sample Sheet

Before importing your sample sheet, the sample sheet must be formatted properly. Create the sample sheet template using the local application on the instrument, or in the cloud using BaseSpace Sequence Hub.

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To create a sample sheet template using one of the local DRAGEN applications on the instrument, refer to the steps in the Set Up DRAGEN Secondary Analysis section and select Export sample sheet in the final step.

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To export a sample sheet for a planned run from BaseSpace Sequence Hub, select Export.

Import Sample Sheet

1.

Select Start.

2.

If you are not signed in, follow the instructions provided in Sign In and Sign Out.

3.

Using the slider bar, select Manual.

4.

Select Import sample sheet for local analysis.

5.

Select Select file and open your sample sheet v2 file. Refer to Format Sample Sheet for information on sample sheet formatting and requirements.

6.

Select Review, and then review your run. Configure the following optional run settings as needed:

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If using custom primers, select the appropriate custom primers checkboxes. Refer to Custom Primers for more information.

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If Read First sequencing is required, deselect the Sequence Indexes First checkbox.

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If the instrument is connected to the cloud, and you are logged in with your BaseSpace Sequence Hub account, select a cloud run setting.

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To use a different output folder from the default, modify the output folder. The default output folder is configured in system settings.

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Modify the Transfer BCL data folder to the external storage and/or cloud checkbox. The default is to transfer files, unless configured differently in system settings.

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Select a custom recipe file.

7.

When finished, refer to Prepare Dry Cartridge.