Run Management

The Planned tab displays runs planned locally or in the cloud. You can plan runs locally on the instrument through Illumina Run Manager. To plan runs in the cloud, use BaseSpace Sequence Hub.

You can edit or delete locally planned runs on the Planned tab. To edit a planned run, select the run on the Planned tab. To delete a planned run, select the ellipsis icon in the Actions column.

The Planned tab displays the following information:

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Status—The status of the sequencing run. Planned runs can exist in one of the following statuses:

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Planned—Run is available to select for sequencing.

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Draft—Run is not available to select for sequencing.

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Needs attention—Run is not available due to an error (eg, cloud connection has been disrupted). Review the error in the Run details screen.

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Run name—The name of the run.

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Application—The DRAGEN secondary analysis applications associated with the run. For more information on installing applications, refer to Applications.

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Last modified—The date and time the run was last edited.

The Active tab displays any in-progress runs. The Active tab includes the date sequencing began, the sequencing status, the % ≥ Q30, yield, and total reads PF metrics.

Select the run name to navigate to the Run details page and view additional details about the run. Select the drop-down next to the run to view additional details on the sequencing status and associated DRAGEN applications.

For more information on run metrics and run status, refer to Monitor Run Progress.

The Completed tab displays runs that have completed sequencing and analysis, were canceled, or failed to complete sequencing or analysis. You can view the location of the sequencing and analysis output data, sequencing metrics, and the amount of instrument data storage consumed by the run. You can view the DRAGEN applications associated with the run, the % ≥ Q30, yield, total reads PF, and the disk space that the run occupies on the instrument. When sequencing data is deleted or transferred off the instrument, the space metric shows 0 GB.

To view additional run results, such as detailed sequencing and secondary analysis metrics, select the run.

The instrument is designed to temporarily store sequencing run data, and completed runs may need to be deleted to create space for subsequent runs.

1.

Select the menu icon in the upper-left corner.

2.

Select Runs.

3.

For the run you want to delete, select the ellipsis icon in the Action column.

4.

Select one of the following options:

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Delete run data—Deletes the sequencing and analysis output folders, but does not remove the run from the Completed tab. You can view the run details, but cannot view the DRAGEN Secondary Analysis report.

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Delete run—Deletes the run data and removes the run from the Completed tab.

5.

In the dialog box, confirm run deletion.

The requeue function is only available for runs that remain on the instrument. Once the data has been deleted from the instrument, it cannot be requeued.

1.

Select the menu icon in the upper-left corner.

2.

Select Runs.

3.

Select the Completed tab.

4.

Select the sequencing run for to be requeued.

5.

Navigate to the Secondary analysis section.

6.

Select Requeue analysis.

7.

Configure requeued analysis settings by following the prompts in the software.

8.

Select Requeue Analysis.