Load Consumables Into the Cartridge

This section is only applicable to protocols utilizing XLEAP-SBS chemistry. If your protocol utilizes standard SBS chemistry, refer to Standard SBS Sequencing Protocol.

This step prepares the cartridge for sequencing by mixing the prefilled reagents and loading diluted libraries and the flow cell.

1. Open the cartridge bag by tearing from the top notch on either side and remove the cartridge from the bag.

Discard the bag and desiccant (moisture-absorbing pouch).

2. Slowly invert the cartridge 10 times to mix reagents.

Internal components can rattle during inversion, which is normal.

3. [XLEAP-SBS Chemistry] Place the cartridge on a flat surface. Lift the cartridge by the arms approximately 1.5–2.5 in (4–7 cm) above the flat surface as shown, and release onto the bench five times.

This process reduces air bubbles and stabilizes cartridge components. If cartridge is accidentally dropped, repeat this process.

1. Open the silver foil package by tearing or cutting with scissors from the top slit on either side.

If unable to use the flow cell immediately, refer to Return Consumables to Storage.

2. Pull the flow cell out of the package.

Set aside the foil package and desiccant in case that you must return the flow cell to storage. The desiccant is contained in a pouch at the bottom of the foil package. Discard them when sequencing begins.

3. Hold the flow cell by the gray tab with the label on the tab facing up.
4. Push to insert the flow cell into the slot on the front of the cartridge.

An audible click indicates that the flow cell is in place. When properly loaded, gray tab protrudes from the cartridge.

5. Pull back and remove the gray tab to expose the flow cell. Recycle the tab.

1. Using a new P1000 pipette tip, pierce the Library reservoir and push the foil to the edges to enlarge the hole.
2. Discard the pipette tip to prevent contamination.
3. Add the diluted library to the bottom of the reservoir by slowly lowering the pipette tip to the bottom of reservoir before dispensing. Avoid touching the foil.
• If using the onboard denature and dilute process, add 20 µl diluted library.
• If using the manual denature and dilute process, add 200 µl diluted library.