System Overview

This section provides an overview of the , including information on hardware, software, and data analysis. It also assembles key concepts and terminology that are embedded throughout the documentation. For detailed specifications, data sheets, applications, and related products, refer to the NextSeq 1000/2000 Sequencing System product page on the Illumina Support Site.

Features

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XLEAP-SBS—The NextSeq 1000/2000 is now capable of performing sequencing runs using XLEAP-SBS chemistry. Use this new generation of sequencing technology only with the compatible software described in the XLEAP-SBS Chemistry Quick Reference.

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Accessibility and reliability—The NextSeq 1000/2000 has local DRAGEN analysis and onboard denature and dilution. To simplify instrument maintenance, the system contains an integrated imaging module and fluidics components are built into the consumables.

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Single-step consumable loading—A single-use cartridge is prefilled with all reagents required for a run. Library and flow cell load directly into the cartridge, which is then loaded onto the instrument. Integrated identification enables accurate tracking.

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NextSeq 1000/2000 software—A suite of integrated software controls instrument operations, processes images, and generates base calls.

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Cloud mode—Plan your run with Run Planning on BaseSpace Sequence Hub. The selected analysis workflow is initiated automatically in the cloud. Run data and analysis results are also provided in the cloud.

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Hybrid mode—Plan your run with Run Planning on BaseSpace Sequence Hub. The selected analysis workflow is then initiated through the on-instrument DRAGEN software.

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Local mode—Plan your run locally with a sample sheet v2 file format. The selected analysis workflow is initiated automatically through the on-instrument DRAGEN software, or manually through BaseSpace Sequence Hub apps after run completion when Proactive, Run Monitoring and Storage is selected.

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Standalone mode—Plan your run without a sample sheet.

Safety Considerations

Review Safety & Compliance before performing any procedures on the system.