XLEAP-SBS Chemistry Quick Reference
This section provides an overview of the ways in which using the XLEAP-SBS chemistry impacts how users interact with the NextSeq 1000/2000 Sequencing System. To use XLEAP-SBS chemistry, there are compatibility requirements and procedural changes that must be followed. Those compatibility requirements and procedural changes are outlined here. For greater detail, refer to XLEAP-SBS Sequencing Protocol.
The following software updates are customer-installable. For installation instructions, refer to their respective Customer Release Notes on the Illumina Support Site.
Compatibility Requirements
Software
| • | NextSeq 1000/2000 Control Software v1.7 or higher. Software installer and installation instructions are on the Software Downloads page of the Illumina Support Site. |
| • | DRAGEN v4.2 or higher. Software installer and installation instructions are on the Software Downloads page of the Illumina Support Site. |
NextSeq 1000/2000 Control Software v 1.7 is only compatible with DRAGEN v4.2. After they are installed, these software updates cannot be reverted.
Reference Genome
| • | DRAGEN v4.2 v9 Reference Genome. Download from the Product Files page on the Illumina Support Site. |
Procedural Changes
Protocol
| • | Loading concentrations and cycle counts have changed. Refer to Sequencing Considerations. |
| • | The required thaw times of XLEAP-SBS consumables are different. Refer to Thaw Consumables. |
| • | Onboard denature and dilute procedures have changed. Refer to Onboard Denature and Dilute. |
| • | Cartridge preparation now includes a step to reduce air bubbles and stabilize cartridge components, and inversion is done slowly. Refer to Prepare the Cartridge. |
