XLEAP-SBS Chemistry Quick Reference
This section provides an overview of the ways in which using the XLEAP-SBS chemistry impacts how users interact with the NextSeq 1000/2000 Sequencing System. To use XLEAP-SBS chemistry, there are compatibility requirements and procedural changes that must be followed. Those compatibility requirements and procedural changes are outlined here. For greater detail, refer to XLEAP-SBS Sequencing Protocol.
The following software updates are customer-installable. For installation instructions for both online and offline installations, refer to the NextSeq 1000/2000 Control Software v1.7 Customer Release Notes on the Illumina Support Site.
Compatibility Requirements
Software
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NextSeq 1000/2000 Control Software v1.7 or higher. Software installer and installation instructions are on the Software Downloads page of the Illumina Support Site. |
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DRAGEN v4.2 or higher. Software installer and installation instructions are on the Software Downloads page of the Illumina Support Site. |
NextSeq 1000/2000 Control Software v1.7 is only compatible with DRAGEN v4.2. Install both software updates at the same time. After they are installed, these software updates cannot be reverted.
Reference Genome
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DRAGEN v4.2 v9 Reference Genome. Download from the Product Files page on the Illumina Support Site. |
Procedural Changes
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Loading concentrations and cycle counts have changed. Refer to XLEAP-SBS Sequencing Considerations. |
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The required thaw times of XLEAP-SBS consumables are different. Refer to Thaw XLEAP-SBS Consumables. |
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Onboard denature and dilute procedures have changed. Refer to Denature and Dilute Protocol Generator. |
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Cartridge preparation now includes a step to reduce air bubbles and stabilize cartridge components, and inversion is done slowly. Refer to Prepare the Cartridge. |
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Q-table binning is updated to reflect the improved sequencing quality and accuracy of XLEAP-SBS chemistry. Refer to Real-Time Analysis Workflow. |
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New custom primer for XLEAP-SBS. Refer to Custom Primers. |
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New dark cycle recipes for XLEAP-SBS. Refer to Dark Cycle Sequencing. |
