Import Custom Library Prep and Index Adapter Kits

You can import custom index adapter kits using the available template.

1.

If the NovaSeq X Series Control Software is open on-instrument, minimize the control software. Refer to Minimize or Exit the Control Software.

2.

Using a browser application on-instrument, access the remote NovaSeq X Series Control Software. For instructions, refer to Use the Remote NovaSeq X Series Control Software .

3.

Open the Menu drop-down in the upper-left corner, and then select Custom kits.

4.

To download the TSV template file, select Download template.

The TSV template file is only available in the remote control software.

5.

Edit the following sections:

Fields must start with an alphanumeric character and can only contain alphanumeric characters, periods, dashes, and underscores.

•

IndexKit—Overview information for the index adapter kit, including name, version, description, and index strategy.

•

Resources—Allows you to provide adapter sequences for Read 1 and Read 2. Based on the values in this section, the imported file sets the index kit type as one of the following options:

•

Standard layout (non-fixed)

•

Fixed layout (single plate)

•

Fixed plate layout (multi plate)

•

Indices—A list of indexes, including name, adapter sequence, and whether the index is for Index 1 or Index 2.

6.

Remove the template instructions included in the angle brackets (< >), and then save the TSV file.

7.

Select Import index adapter kit, navigate to the custom index adapter kit (*.tsv), and select Open.

8.

After successfully importing the custom index adapter kit, select the name to review and edit information.

Use the following instructions to upload custom library prep kits.

1.

Select the instrument icon to open the global navigation menu.

2.

Select Settings, and then select Custom kits.

3.

Select Add library prep kit, and enter the following information:

•

Library prep kit name

•

[Optional] Description

•

[Optional] Organization. The company or institution that owns the custom library prep kit. The organization cannot be Illumina.

•

Allowed read types

•

Default read type

•

Default read cycle

4.

From the drop-down list, select at least one compatible index adapter kit.

5.

Select Save.

6.

After successfully adding the library prep kit, select the name to review and edit information.