Initiate a Sequencing Run

You can initiate a sequencing run by selecting a planned run or by creating a manual run. For information on planning a sequencing run, refer to Plan a Sequencing Run. If analyzing data in the cloud, secondary analysis begins automatically in BaseSpace Sequence Hub or ICA. If analyzing data locally, on-instrument analysis begins automatically and output files are stored in the selected output folder.

Storage requirements are affected by the number of sequencing cycles, size of the samples, secondary analysis configuration, and compression type. The NovaSeq X Series can store at least 2 flow cells worth of 25B data for any secondary analysis configuration. For secondary analysis configurations that generate smaller amounts of data (for example, DRAGEN BCL Convert or DRAGEN Enrichment), the sequencing system might be able to store 4 flow cells worth of 25B data.

If the storage is not sufficient to start a run, an error message prompts you to clear space. For more information, refer to Clear Hard Drive Space.

For example data output for each flow cell type, refer to Illumina Instrument Control Computer Security and Networking.