Plan a Sequencing Run

You can use one of the following options to plan a sequencing run for the NovaSeq X Series. After setting up a run, the planned run displays on the Planned tab in the Runs screen and is available for selection when initiating a sequencing run.

To plan your run in the cloud, use BaseSpace Sequence Hub.
Before planning a run, make sure you have configured your cloud settings. Refer to Configure Cloud Settings and Proactive Support for more information.
After saving, the run is available on the instrument for sequencing. If the instrument is not connected to the cloud, you can export a sample sheet v2 file and import the sequencing run into your sequencing system.
For more information on BaseSpace Sequence Hub, refer to BaseSpace Sequence Hub support site page.
To plan your run locally, use the NovaSeq X Series Control Software available on-instrument or via a networked computer.
After sequencing, on-instrument analysis starts automatically. CBCL data and DRAGEN secondary analysis output files are stored in the selected output folder.
If using a networked computer, the computer must be connected to the same local network as the sequencing system and have a root certificate installed.
To set up a run and perform sequencing without a network connection, refer to Start a Manual Run.