Lab Setup for PCR Procedures

Some library prep methods require the polymerase chain reaction (PCR) process.

Establish dedicated areas and lab procedures to prevent PCR product contamination before you begin work in the lab. PCR products can contaminate reagents, instruments, and samples, delaying normal operations and causing inaccurate results.

Pre-PCR and Post-PCR Areas

Use the following guidelines to avoid cross-contamination.

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Establish a pre-PCR area for pre-PCR processes.

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Establish a post-PCR area for processing PCR products.

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Do not use the same sink to wash pre-PCR and post-PCR materials.

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Do not use the same water purification system for pre-PCR and post-PCR areas.

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Store supplies used for pre-PCR protocols in the pre-PCR area. Transfer them to the post-PCR area as needed.

Dedicate Equipment and Supplies

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Do not share equipment and supplies between pre-PCR and post-PCR processes. Dedicate a separate set of equipment and supplies in each area.

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Establish dedicated storage areas for consumables used in each area. For storage temperature requirements and consumable dimensions, refer to Sequencing Consumables.