Protocol
This section describes the Illumina 5-Base DNA Prep with Enrichment protocol and provides instructions for preparing gDNA and cfDNA input.
The Illumina 5-Base DNA Prep with Enrichment kit includes reagents to hybridize four libraries within a single enrichment reaction. Hybridization binds enrichment probe oligonucleotides to targeted regions of the methylation-converted libraries.
Use the procedures and reagents appropriate for your input type. After completing input preparation, continue the protocol with Prepare Reagents.
Before you begin, do as follows.
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Review the entire protocol. |
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Review the preparation sections appropriate for your input type. |
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Before proceeding, confirm kit contents and make sure that you have the required equipment and consumables. This protocol requires library prep reagents and indexes. For details, refer to Product Contents. |
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Have ice available. The library prep PCR plate and many of the consumables must be placed on ice during the protocol. |
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Follow the protocol in the order shown, using the specified volumes and incubation parameters. |
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Unless a safe stopping point is specified in the protocol, proceed immediately to the next step. |
Illumina 5-Base DNA Prep with Enrichment Kit Workflow
The following diagram illustrates the Illumina 5-Base DNA Prep with Enrichment kit workflow.
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Each protocol section starts with Prepare Reagents and ends at a safe stopping point. |
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Time estimates are based on preparing 24 samples using a multichannel pipette. |
