User-Supplied Consumables & Equipment

The following sections provide information on the required user-supplied consumables and equipment.

Item

Supplier

0.2 ml PCR 8-tube strips and caps or PCR reaction plates (if you have a compatible magnetic bead rack)

General lab supplier

Absolute ethanol, molecular biology grade

General lab supplier

Nuclease-free 1X low EDTA TE Buffer (IDTE), pH 8.0, 10 mM Tris, 0.1 mM EDTA

IDT, catalog # 11-05-01-09 or general lab supplier

Nuclease-free water

Thermo Fisher Scientific, catalog # AM9937 or general lab supplier

Pipette tips, filtered, low retention, sterilized, 20 μl

One of the following suppliers:

• Rainin, catalog # 30389226
• Filtrous, catalog # PTF-LS-0020
• VWR, catalog #76322-528

Pipette tips, filtered, low retention, sterilized, 200 μl

One of the following suppliers:

• Rainin, catalog # 30389240
• Filtrous, catalog # PTF-LS-0200
• VWR; catalog # 76322-150

Pipette tips, filtered, low retention, sterilized, 1000 μl

One of the following suppliers:

• Rainin, catalog # 30389213
• Filtrous, catalog # PTF-LS-1000
• VWR, catalog # 76322-154

Oligonucleotide additive primers and associated index primers (Refer to Oligonucleotides)

Integrated DNA Technologies

Quantification consumables, any of the following:

• [Bioanalyzer] Agilent High Sensitivity DNA kit
• [Tapestation] HS-D5000 for cDNA and HS-D1000 for NGS library

One of the following suppliers, depending on system:

• Agilent, catalog # 5067-4626,
• Agilent, catalog # HS-D5000 5067-5592 and 5067-5593
• Agilent, catalog # HS-D1000 5067-5584 and 5067-5585

Qubit 1X dsDNA High Sensitivity Assay Kit

Thermo Fisher Scientific, catalog #Q33230 or Q33231

Qubit Assay Tubes

Thermo Fisher Scientific, catalog # Q32856

Description

Supplier

0.2 ml/1.5 ml Magnetic Separation Combo Rack

Permagen, MSR1224B, or equivalent from alternative supplier

Benchtop mini microcentrifuge 2000 × g, suitable for 1.5 ml tubes, 0.5 ml tubes and PCR tube strips

Thermo Fisher, catalog # 75004061, or general lab supplier

Micropipettes, 1–1000 µl capabilities

General lab supplier

Quantification system, either of the following:

• 2100 Bioanalyzer Desktop System
• 4200 Tapestation System

One of the following suppliers, depending on system:

• Agilent, catalog # G2940CA
• Agilent, catalog # G2991BA

Qubit Fluorometer

Thermo Fisher Scientific

Thermal cycler

General lab supplier

Vortexer

General lab supplier