User-Supplied Consumables & Equipment

0.2 ml PCR 8-tube strips or PCR reaction plates for library preparation (if you have a compatible magnetic bead rack).

5 ml centrifuge tubes, sterile and PCR Clean

Absolute ethanol, molecular biology grade

Nuclease-free 1X low EDTA TE Buffer (IDTE), pH 8.0, 10 mM Tris, 0.1 mM EDTA

Nuclease-free water

Pipette tips, filtered, low retention, sterilized, 20 μl

Pipette tips, filtered, low retention, sterilized, 200 μl

Pipette tips, filtered, low retention, sterilized, 1000 μl

Oligonucleotide primers (Refer to Oligonucleotides)

Quantification consumables, any of the following:

Qubit 1X dsDNA High Sensitivity Assay Kit

3 x 15 ml/50 ml Magnetic Separation Combo Rack

5 ml tube stand

Benchtop centrifuge with swinging bucket rotor, suitable for 5 ml tubes

Benchtop mini microcentrifuge 2000 × g, suitable for 1.5 ml tubes, 0.5 ml tubes and PCR tube strips.

Magnetic separation rack suitable for PCR tube strips

Micropipettes, 1–1000 µl

Quantification system, either of the following: 

Qubit Fluorometer

Thermal cycler