User-Supplied Consumables & Equipment

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Consumable

Supplier

0.5 ml microcentrifuge tubes, nuclease-free

General lab supplier

1.7 ml microcentrifuge tubes, nuclease-free

General lab supplier

2.0 ml DNA LoBind tubes, nuclease-free

Eppendorf, catalog # 022431048

Conical centrifuge tubes (15 ml or 50 ml)

General lab supplier

2 µl aerosol resistant pipette tips

General lab supplier

10 µl aerosol resistant pipette tips

General lab supplier

20 µl aerosol resistant pipette tips

General lab supplier

200 µl aerosol resistant pipette tips

General lab supplier

1000 µl aerosol resistant pipette tips

General lab supplier

Eppendorf twin.tec PCR Plate 96, semiskirted

Eppendorf, catalog # 951020303

Eppendorf twin.tec PCR Plate 96, LoBind

Eppendorf, catalog # 0030129504

Nuclease-free reagent reservoirs

General lab supplier

Microseal 'B' adhesive seals

Bio-Rad, catalog # MSB-1001

RNase/DNase-free water

General lab supplier

Ethanol (200 proof for molecular biology)

Sigma-Aldrich, product # E7023

Illumina TruSight FFPE QC kit

Illumina catalog # 20139070

TE buffer (TEB)

(10 mM Tris-HCl, 1 mM EDTA pH 8)

General lab supplier

[Library Normalization] Qubit dsDNA BR Assay Kit

Thermo Fisher Scientific, catalog # Q32850 or Q32853

[Library Normalization] Qubit Assay Tubes

Thermo Fisher Scientific, catalog # Q32856

Equipment

Supplier

Thermal cycler. One of the following:

• Bio-Rad C1000 Touch Thermal Cycler with 96-Deep Well Reaction Module*
• Thermal cycler with the following specifications:
• Heated lid
• Sample Volume at least 100 µl. (Deep well block recommended for best performance.)
• Ramp rate no less than 2.5°C/sec
• Temperature range 0°C to 100°C
• Accuracy ≤ ± 0.25°C
• Uniformity < 0.5°C
• Accepts Eppendorf twin.tec PCR plate 96, semiskirted

 

• Bio-Rad, part # 1851197*, or equivalent.
• General supplier

Magnetic stand with bar configuration:

• DynaMag-96 Side Skirted Magnet

Thermo Fisher Scientific, catalog # 12027

Microplate centrifuge

General lab supplier

Microcentrifuge

General lab supplier

Plate shaker (BioShake iQ) with 96 well PCR plate adapter

Q Instruments, catalog # 1808-0506 and 1808–1041

Ultrasonicator with associated accessories.

(Refer to Fragment FFPE DNA for optimization of ultrasonicators.)

General lab supplier

Vortexer

General lab supplier

[Optional] Agilent DNA ScreenTape Analysis (D1000)

(Refer to DNA Shearing Recommendations.)

Agilent part # 5067-5584

[Optional] Agilent 4200 TapeStation System

(Refer to DNA Shearing Recommendations.)

Agilent part # G2991BA

* End of life announced. Refer to vendor site for more information.

Equipment

Supplier

Thermal cycler. One of the following:

• Bio-Rad C1000 Touch Thermal Cycler with 96-Deep Well Reaction Module*
• Thermal cycler with the following specifications:
• Heated lid
• Sample Volume at least 100 µl. (Deep well block recommended for best performance.)
• Ramp rate no less than 2.5°C/sec
• Temperature range 0°C to 100°C
• Accuracy ≤ ± 0.25°C
• Uniformity < 0.5°C
• Accepts Eppendorf twin.tec PCR plate 96, semiskirted

 

• Bio-Rad, part # 1851197*, or equivalent.
• General supplier

Magnetic stands (peg and bar configuration):

• Bar configuration: DynaMag-96 Side Skirted Magnet
• Peg configuration: Magnetic Stand-96

 

• Thermo Fisher Scientific, catalog # 12027
• Thermo Fisher Scientific, catalog # AM10027

Microplate centrifuge

General lab supplier

Microcentrifuge

General lab supplier

Plate shaker (BioShake iQ) with adapter plates:

• Flat bottom standard microplate adapter
• 96 well PCR plate adapter

Q Instruments, catalog # 1808-0506

• # 1808-1021, bead-based normalization option only
• # 1808-1041

[Manual Normalization] Qubit Fluorometer 4.0

Thermo Fisher Scientific, catalog # Q33238

Vortexer

General lab supplier

* End of life announced. Refer to vendor site for more information.