Product Contents

Make sure that you have all reagents identified in this section before starting library prep. The protocol requires one Illumina Stranded mRNA Prep, Ligation kit and at least one set of lllumina RNA UD Indexes. Combine all four sets to index up to 384 libraries.

Component

Kit Name

Illumina Catalog #

Library prep

Illumina Stranded mRNA Prep, Ligation, Ligation (16 Samples)

20040532

Illumina Stranded mRNA Prep, Ligation, Ligation (96 Samples)

20040534

Index adapters

lllumina RNA UD Indexes Set A, Ligation (96 Indexes, 96 Samples)

20091655

lllumina RNA UD Indexes Set B, Ligation (96 Indexes, 96 Samples)

20091657

lllumina RNA UD Indexes Set C, Ligation (96 Indexes, 96 Samples)

20091659

lllumina RNA UD Indexes Set D, Ligation (96 Indexes, 96 Samples)

20091661

IDT for Illumina RNA UD Indexes Set A, Ligation (96 Indexes, 96 Samples)

20040553

IDT for Illumina RNA UD Indexes Set B, Ligation (96 Indexes, 96 Samples)

20040554

IDT for Illumina RNA UD Indexes Set C, Ligation (96 Indexes, 96 Samples)

20040555

IDT for Illumina RNA UD Indexes Set D, Ligation (96 Indexes, 96 Samples)

20040556

The prep kits provide reagents for mRNA selection, cDNA synthesis, and library prep in a 16- or 96-sample size. The indexes sets provide premixed Index 1 (i7) and Index 2 (i5) adapters with the adapters necessary for ligation.

The Illumina kits do not include Agencourt AMPure XP beads. For supplier information, refer to User-Supplied Consumables & Equipment.

Illumina PolyA Capture

Quantity

Reagent

Description

Cap

Shipment

Storage

1

BBB

Bead Binding Buffer

Clear

2°C to 8°C

2°C to 8°C

1

BWB

Bead Washing Buffer

Yellow

2°C to 8°C

2°C to 8°C

3

ELB

Elution Buffer

Clear

2°C to 8°C

2°C to 8°C

1

RPBX

RNA Purification Beads

Clear

2°C to 8°C

2°C to 8°C

Illumina cDNA Synthesis

Quantity

Reagent

Description

Cap

Shipment

Storage

1

EPH3

Elute, Prime, Fragment 3HC Mix

Clear

‑25°C to ‑15°C

‑25°C to ‑15°C

1

FSA

First Strand Synthesis Mix

Amber

‑25°C to ‑15°C

‑25°C to ‑15°C

1

RSB

Resuspension Buffer

Clear

‑25°C to ‑15°C

‑25°C to ‑15°C

1

RVT

Reverse Transcriptase

Clear

‑25°C to ‑15°C

‑25°C to ‑15°C

1

SMM

Second Strand Marking Master Mix

Clear

‑25°C to ‑15°C

‑25°C to ‑15°C

Illumina RNA Prep, Ligation

Quantity

Reagent

Description

Cap

Shipment

Storage

1

ATL4

A-Tailing Mix

Clear

-25°C to -15°C

-25°C to -15°C

1

EPM

Enhanced PCR Mix

Clear

-25°C to -15°C

-25°C to -15°C

1

LIGX

Ligation Mix

Clear

-25°C to -15°C

-25°C to -15°C

1

RSB

Resuspension Buffer

Clear

-25°C to -15°C

-25°C to -15°C

1

STL

Stop Ligation Buffer

Red

-25°C to -15°C

-25°C to -15°C

Illumina PolyA Capture

Quantity

Reagent

Description

Cap

Shipment

Storage

1

BBB

Bead Binding Buffer

Clear

2°C to 8°C

2°C to 8°C

1

BWB

Bead Washing Buffer

Clear

2°C to 8°C

2°C to 8°C

3

ELB

Elution Buffer

Clear

2°C to 8°C

2°C to 8°C

4

RPBX

RNA Purification Beads

Clear

2°C to 8°C

2°C to 8°C

Illumina cDNA Synthesis

Quantity

Reagent

Description

Cap

Shipment

Storage

4

EPH3

Elute, Prime, Fragment 3HC Mix

Clear

-25°C to -15°C

-25°C to -15°C

4

FSA

First Strand Synthesis Mix

Amber

-25°C to -15°C

-25°C to -15°C

2

RSB

Resuspension Buffer

Clear

-25°C to -15°C

-25°C to -15°C

1

RVT

Reverse Transcriptase

Clear

-25°C to -15°C

-25°C to -15°C

4

SMM

Second Strand Marking Master Mix

Clear

-25°C to -15°C

-25°C to -15°C

Illumina RNA Prep, Ligation

Quantity

Reagent

Description

Cap Color

Shipment

Storage

4

ATL4

A-Tailing Mix

Clear

-25°C to -15°C

-25°C to -15°C

4

EPM

Enhanced PCR Mix

Clear

-25°C to -15°C

-25°C to -15°C

4

LIGX

Ligation Mix

Clear

-25°C to -15°C

-25°C to -15°C

4

RSB

Resuspension Buffer

Clear

-25°C to -15°C

-25°C to -15°C

4

STL

Stop Ligation Buffer

Red

-25°C to -15°C

-25°C to -15°C

Each kit contains one plate of Illumina RNA Index Anchors and one plate of lllumina RNA UD Indexes, Ligation Set A, B, C, or D.

For index adapter sequences, refer to Illumina Adapter Sequences.

Description

Shipment

Storage

lllumina RNA UD Indexes Set A, Ligation (UDP0001–UDP0096)

-25°C to -15°C

-25°C to -15°C

lllumina RNA UD Indexes Set B, Ligation (UDP0097–UDP0192)

-25°C to -15°C

-25°C to -15°C

lllumina RNA UD Indexes Set C, Ligation (UDP0193–UDP0288)

-25°C to -15°C

-25°C to -15°C

lllumina RNA UD Indexes Set D, Ligation (UDP0289–UDP0384)

-25°C to -15°C

-25°C to -15°C

Each kit contains one plate of IDT for Illumina RNA Index Anchors and one plate of IDT for Illumina DNA/RNA UD Indexes Set A, B, C, or D.

For index adapter sequences, refer to Illumina Adapter Sequences.

Description

Shipment

Storage

IDT for lllumina RNA UD Indexes Set A, Ligation (UDP0001–UDP0096)

-25°C to -15°C

-25°C to -15°C

IDT for lllumina RNA UD Indexes Set B, Ligation (UDP0097–UDP0192)

-25°C to -15°C

-25°C to -15°C

IDT for lllumina RNA UD Indexes Set C, Ligation (UDP0193–UDP0288)

-25°C to -15°C

-25°C to -15°C

IDT for lllumina RNA UD Indexes Set D, Ligation (UDP0289–UDP0384)

-25°C to -15°C

-25°C to -15°C