Protocol

This section describes the Nextera XT DNA protocol.

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Review the planned complete sequencing workflow, from sample through analysis, to ensure compatibility of products and experiment parameters.

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Before proceeding, confirm kit contents and make sure that you have the required components, equipment, and consumables. This protocol requires library prep reagents and index adapters. Index adapters are sold separately. Refer to Illumina-Supplied Consumables.

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Follow the protocols in the order shown, using the specified volumes and incubation parameters.

Revision History

Document # 15031942 v07

For Research Use Only. Not for use in diagnostic procedures (except as specifically noted).

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Nextera XT DNA Library Prep

nextera-xt-dna

15031942_07_nextera-xt-product-documentation.pdf