Product Contents

The protocol requires one capture and barcoding kit, one library preparation kit, and one equipment kit.

Component

Kit Name

Illumina Catalog #

Library prep with capture and barcoding

Illumina Single Cell 3′ RNA Prep, T20 (4 samples, 20,000 cells/sample)

20135692

Single cell capture and barcoding only

Illumina Single Cell 3′ RNA Capture, T20 (4 samples, 20,000 cells/sample)

20132792

Library prep only

Illumina Single Cell Library Prep (8 reactions)

20132789

[Optional] Index adapters

Illumina Single Cell Unique Dual Indexes (96 Indexes, 96 Samples)

20132788

Starter equipment

Illumina Single Cell Prep Starter Equipment

20132796
Illumina Single Cell 3′ RNA Capture, T20, Box 1, Store at 15°C to 30°C

Quantity

Reagent

Description

2

PAR

Partitioning Reagent

1

CLB3

Chemical Lysis Buffer 3

1

DPR

Departitioning Reagent

Illumina Single Cell 3′ RNA Capture, T20, Box 2, Store at 2°C to 8°C

Quantity

Reagent

Description

1

IPB2

Illumina Purification Beads 2

1

BBF

Breaking Buffer

4

PWB1

Washing Buffer

1

CEB

cDNA Extraction Buffer

Illumina Single Cell 3′ RNA Capture, T20, Box 3, Store at -25°C to -15°C

Quantity

Reagent

Description

1

NSB

Nuclei Suspension Buffer

1

RTEM

RT Enzyme Mix

1

RTAM

RT Additive Mix

1

PMM4

4X PCR Master Mix

1

WTAP

WTA Primer

1

RNI

RNase Inhibitor (40 U/μl)

1

CSB

Cell Suspension Buffer

1

BSA

25% BSA

Illumina Single Cell 3′ RNA Capture, T20, Box 4, Store at -85°C to -65°C

Quantity

Reagent

Description

4

PIP

T20 PIPs

4

TSO1

Template-Switch Oligo
Illumina Single Cell 3′ RNA Capture, T20, Accessories Bag

Quantity

Component

8

3 ml syringe

8

G22 blunt-bottom syringe needle
Illumina Single Cell Library Prep, Box 1, Store at 2°C to 8°C

Quantity

Reagent

Description

1

IPB2

Illumina Purification Beads 2

1

NFW

Nuclease-Free Water
Illumina Single Cell Library Prep, Box 2, Store at -25°C to -15°C

Quantity

Reagent

Description

1

LPB

Library Prep Buffer

1

LPE

Library Prep Enzymes

1

LPMA

Library Prep Mix A

1

LAM

Library Adapter Mix

1

PMM4

4X PCR Master Mix

1

UDI8

Illumina Single Cell Prep UDI Mix Strip

Quantity

Component Name

Used for Kit size

1

PIPseq Vortex Mixer

All

1

PIPseq rotating vortex assembly for 5 ml tubes

T100

1

PIPseq rotating vortex assembly for 1.5 ml tubes

T20

1

PIPseq rotating vortex assembly for 0.5 ml tubes

T2 and T10

2

Rotating vortex base assembly

All

1

User manual for PIPseq Vortex Mixer

All

1

US Power Supplies for Vortex Mixer

All

1

Vortexer gap cover

All

2

PIPseq Combination 1.5 ml and 0.5 ml tube stand, blue

T2,T10,T20

1

PIPseq 8-tube stand, for 0.2 ml tubes, black

T2

1

PIPseq guide rack, red

T2

2

International Plug Converter

All

1

PIPseq Dry Bath 5 ml block

T100

1

PIPseq Dry Bath 1.5 ml block

T20

1

PIPseq Dry Bath 0.5 ml block

T2 and T10

1

US Power Supplies for PIPseq Dry Bath

All

1

User Manual for PIPseq Dry Bath

All

1

PIPseq Dry Bath with heated lid, which includes the following materials:

• 2.5 mm Allen key wrench, silver
• PIPseq Dry Bath Stylus
• Replacement tips for PIPseq Dry Bath Stylus
• 5x20 mm 5A 250V Fast blow fuse for PIPseq Dry Bath
• Dry block removal tool, white

All