VeriSeq NIPT Batch Manager
The VeriSeq NIPT Batch Manager manages the status of samples, batches, and pools through the user interface. The system allows sample tracking across multiple liquid handling systems and sequencing instruments systems, and through the analysis pipeline. For more information about sample processing procedures, refer to the Protocol section.
You can manage samples within the workflow through three different categories, referred to as objects. These objects are described in the following table.
|
Object |
Description |
|---|---|
|
Sample |
Result of a one-time draw of 1 ml of plasma from a single blood tube. Samples are associated with the barcode of the blood tube (the sample barcode) and the batch. |
|
Batch |
Plate of 24, 48, or 96 samples processed through the cfDNA Extraction and Library Prep process. |
|
Pool |
Normalized and diluted volume of instrument-ready, dual-index libraries. Each pool contains up to 48 samples. |
The following table describes the actions that can be applied to objects during processing.
|
Action |
Object |
Report Generated |
Description |
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|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
Invalidation
|
Sample |
Sample Invalidation |
Sample flagged by the user as no longer valid for processing. No data is generated for invalidated samples. Example: Visible blood cell carryover during Plasma Isolation. |
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|
Batch |
Batch Invalidation |
Batch flagged by the user as no longer valid. If batch invalidation occurs before pool generation, all samples are invalidated. Example: Dropped or otherwise improperly handled plate. |
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|
Pool |
Pool Invalidation |
Pool flagged by the user as no longer valid. After two pool invalidations, all samples within the pool are invalidated. Example: Entire pool volume used during two sequencing failures. |
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|
QC Failure
|
Sample |
Sample Invalidation |
VeriSeq NIPT Solution v2 – RUO automatically flagged sample as invalid due to the failure of a specified Quality Control (QC) metric or from a system-detected liquid handling failure. |
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|
Batch |
Batch Invalidation |
VeriSeq NIPT Solution v2 – RUO automatically flagged entire batch as invalid. Example: System failure during liquid handling. |
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|
Cancellation |
Sample |
Sample Cancellation |
Laboratory management flagged sample as canceled. No data is generated. |
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Edit Sample Attributes
|
Sample |
Sex Reporting |
Sex reporting flagged by the user as Yes, No, or SCA.
|
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|
Sample |
Sample Type |
Sample type flagged by the user as Singleton, Twin, Control, or No Template Control (NTC). The designation of the sample type directly affects the analysis of the assay. To ensure accurate data, the sample type is required to be accurate. |
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|
Sample |
Screen Type |
Screen type flagged by the user as basic (21, 18, 13, X, and Y only) or genomewide (all chromosomes). |
After an invalidation, QC failure, or cancellation action, the object is not processed further. Laboratory information management systems (LIMS) can use Sample Invalidation reports to indicate sample reprocessing from the blood collection tube.
