Unrecoverable Error Notifications
Unrecoverable errors are conditions that reach a terminal state where no further action can resume the assay execution.
|
Notification |
Step |
When |
Alert Level |
|
Recommended Action |
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|---|---|---|---|---|---|---|---|---|---|---|---|
|
Batch Failure |
Library Prep |
Batch QC failed. |
Notice |
Yes |
Restart library plating. |
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|
Report Generating Failure |
Reporting |
The system failed to generate a report. |
Alert |
Yes |
|
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|
Failed to Parse Run Parameters file |
Sequencing |
The system was not able to open/parse the RunParameters.xml file. |
Warning |
Yes |
The RunParameters.xml file is corrupted. Check the instrument configuration and resequence the pool. |
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|
Unrecognized Run Parameters |
Sequencing |
The software read Run Parameters that are not compatible. |
Warning |
Yes |
The software is unable to construct sequencing run parameters from the instrument configuration file. Check the instrument configuration and resequence the pool. |
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|
Invalid Run Parameters |
Sequencing |
The software read required Run Parameters that are not compatible with the assay. |
Warning |
Yes |
The software compatibility check failed. Check the instrument configuration and resequence the pool.
|
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|
No Pool Barcode found |
Sequencing |
The software was not able to associate the flow cell for the sequencing run with a known pool barcode. |
Warning |
Yes |
Possible incorrect pool barcode entry. Resequence the pool. |
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|
Sequencing Completed but Pool Barcode File Missing |
Sequencing |
The sequencing run was completed but the file containing the pool barcode was not detected. |
Alert |
Yes |
Possible sequencer failure. Contact IlluminaTechnical Support for assistance. |
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|
Unable to read Pool Barcode File |
Sequencing |
The file containing the pool barcode is corrupted. |
Alert |
Yes |
Possible sequencer or network failure. Contact Illumina Technical Support for assistance. |
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|
Pool Barcode File Mismatch |
Sequencing |
The detected pool barcode file references a different flow cell ID than the one associated with the sequencing run. |
Alert |
Yes |
Possible sequencer failure. Contact Illumina Technical Support for assistance. |
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|
Sequencing Timed Out |
Sequencing |
The sequencing run has not completed in a given timeframe. |
Warning |
Yes |
Check the sequencer and the network connection. Resequence the pool. |
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|
Sequencing QC files generation failed |
Sequencing QC |
The sequencing run has completed but the interop QC files are corrupted. |
Alert |
Yes |
Check the sequencer, and the network connection. Resequence the pool. |
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|
Sequencing QC failed |
Sequencing QC |
The sequencing run has completed and the sequencing QC check failed. |
Notice |
Yes |
Resequence the pool. |
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|
Analysis Failed for Maximum number of attempts |
Analysis |
All Analysis attempts have failed. Will not retry. |
Warning |
Yes |
Resequence the second pool. |
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|
Analysis Post-Processing Failed |
Post-Analysis |
The software failed to post process the analysis results. |
Alert |
Yes |
|
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|
Plate Level Contamination Detected |
Analysis |
The software has detected a plate level contamination/admixture event. |
Notice |
Yes |
|
