Set Up a Manual Run

Use the Manual run setup option to enter run information manually. No on-instrument secondary analysis is performed.

1. From the Home screen, select Sequence.
2. From the Sequence Mode Selection screen, select Manual.
3. [Optional] From the BaseSpace Sequence Hub Options screen, select Use BaseSpace Sequence Hub for this run.

For more information, refer to Send Run Data to BaseSpace Sequence Hub [Optional].

4. Select Next.
5. Set the Read Type, Read Length and Custom Primers for all of your cycles. Enter up to 10 cycles for each Index Read.
6. Select Next to proceed to Load the Flow Cell.

Clean flow cells before loading. For more information, refer to Clean the Flow Cell.