Plan a Sequencing Run

You can use one of the following options to plan a sequencing run for the NovaSeq X Series. After setting up a run, the planned run displays on the Planned tab in the Runs screen and is available for selection when initiating a sequencing run.

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To plan your run in the cloud, use BaseSpace Sequence Hub.

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Before planning a run, make sure you have configured your cloud settings. Refer to Configure Cloud Settings and Illumina Proactive Support for more information.

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After saving, the run is available on the instrument for sequencing. If the instrument is not connected to the cloud, you can export a sample sheet v2 file and import the sequencing run into your sequencing system.

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For more information on BaseSpace Sequence Hub, refer to BaseSpace Sequence Hub support site page.

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To plan your run locally, use the NovaSeq X Series Control Software available on-instrument or via a networked computer.

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After sequencing, on-instrument analysis starts automatically. CBCL data and DRAGEN secondary analysis output files are stored in the selected output folder.

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If using a networked computer, the computer must be connected to the same local network as the sequencing system and have a root certificate installed.

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To set up a run and perform sequencing without a network connection, refer to Start a Manual Run or Import Sample Sheet.