Custom Primers

Follow the run setup instructions described in the Run Settings. Using custom primers for a run on an Illumina iSeq 100 Sequencing System requires three additional steps at the run setup:

Set the iSeq 100 to manual run mode and enable the custom primers setting.
Select the custom primers in the control software during run setup.
Prepare and add the appropriate volume of each custom primer to the library reservoir of the cartridge after pre-run checks are completed.

To use custom primers with the iSeq 100 i1 Reagent v2 kits, update and install iSeq 100 Control Software v3.0 or later. The iSeq 100 Sequencing System support pages on the Illumina support center provides additional resources.

The iSeq 100 allows the use of two custom read primers and two custom index primer combinations. Each primer is loaded into the library reservoir individually.

Depending on your library preparation kit, you might be required to use Illumina primer mixes. Refer to VP10 and VP14 Custom Primers for more information.

Illumina cannot guarantee the performance or compatibility of custom primers. You are responsible for validating custom primers for sequencing on the iSeq 100 Sequencing System.