Load Consumables Into the Cartridge

1. [Optional] To open an instructional video on cartridge preparation and loading, select Sequence.
2. Open the cartridge bag from the notches.
3. Avoiding the access window on top of the cartridge, remove the cartridge from the bag. Discard the bag.
4. Invert the cartridge five times to mix reagents.

Internal components can rattle during inversion, which is normal.

5. Tap the cartridge (label facing up) on the bench or other hard surface five times to ensure reagent aspiration.
6. If you are not using custom primers, proceed to Load Library. If you are using custom primers, refer to Custom Primers.
1. Using a new pipette tip, pierce the library reservoir and push the foil to the edges to enlarge the hole.

2. Discard the pipette tip to prevent contamination.
3. Add 20 µl diluted library to the bottom of the reservoir. Avoid touching the foil.

If you are using custom primers, add the custom primers before the diluted library. Refer to Custom Primers for more information.

1. Open the white foil flow cell package from the notches. Use within 24 hours of opening.
2. Pull the flow cell out of the package.
• Touch only the plastic when handling the flow cell.
• Avoid touching the electrical interface, CMOS sensor, glass, and gaskets on either side of the glass.

3. Hold the flow cell by the grip points with the label facing up.
4. Insert the flow cell into the slot on the front of the cartridge.

An audible click indicates that the flow cell is in place. When properly loaded, the grip protrudes from the cartridge and the glass is visible from the access window.

  1. Loading the flow cell
  2. Loaded flow cell
5. Dispose of the packaging as follows.
a. Remove the clamshell case from the foil package.
b. Remove the desiccant from the clamshell case.
c. Recycle the clamshell case, and discard the foil package and desiccant.
6. Proceed depending on whether the system is integrated with Local Run Manager:
• If using Local Run Manager, refer to Set Up a Sequencing Run (Local Run Manager).
• If not using Local Run Manager, refer to Set Up a Sequencing Run (Manual Mode).